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J. Renewable Sustainable Energy 1, 063101 (2009); doi:10.1063/1.3256191 (8 pages)

Design an immobilized lipase enzyme for biodiesel production

Kenthorai Jegannathan ,
Leong Jun-Yee ,
Eng-Seng Chan ,
and Pogaku Ravindra

Centre of Materials and Minerals, School of Engineering and Information Technology, Universiti Malaysia Sabah, Kota Kinabalu, Sabah 88999, Malaysia Map This map

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In this study, an eco-friendly immobilized lipase was designed for biodiesel production process. Lipase enzyme was encapsulated in κ-carrageenan by coextrusion technique. The various parameters, such as catalytic activity, stability, reusability, shape, and size of the encapsulated lipase, were studied to design an immobilized lipase for biodiesel production. Transesterification of palm oil with methanol was used for biodiesel production. At the optimum conditions, the results were found to be quite promising, converting the raw material near to 100% biodiesel production. This attempt was found to be very effective and eco-friendly with the environmentally benign novel technique.

© 2009 American Institute of Physics

Article Outline

  1. INTRODUCTION
  2. EXPERIMENTAL SECTION
    1. Materials
    2. Lipase immobilization
    3. Transesterification process parameters
    4. Sampling and analysis
  3. RESULTS AND DISCUSSIONS
    1. Lipase immobilization
      1. Encapsulation with coextrusion technique
      2. Selection of lipase
      3. The choice of immobilization matrix
      4. Fixed conditions
    2. Catalytic function
    3. Noncatalytic function
      1. Isolation of catalyst from the reaction mixture
      2. Stability
      3. Reusability
      4. Eco-friendly function
  4. CONCLUSIONS

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KEYWORDS and PACS

Keywords

biofuel, biotechnology, enzymes, fuel processing industries

PACS

ARTICLE DATA

History
Received 22 July 2009
Accepted 7 October 2009
Published 5 November 2009
Permalink
http://link.aip.org/link/JRSEBH/v1/i6/p063101/s1

PUBLICATION DATA

ISSN:

19417012 (print)  
19417012 (online)

Publisher:

$abstract.society.value is a Member of CrossRef American Institute of Physics

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Figures (4) Tables (2)

Figures (click on thumbnails to view enlargements)

FIG. 1
Schematic diagram of encapsulation procedure. From Jegannathan, Chan, and Ravindra, J. Mol. Catal., B Enzym, 58, 78 (2009). Copyright © 2009, by Elsevier.
FIG. 1 View Enlargement | Download High Resolution Image (.zip file)
FIG. 2
Production of methyl ester (biodiesel) variation with reaction time.
FIG. 2 View Enlargement | Download High Resolution Image (.zip file)
FIG. 3
Picture of κ-carrageenan encapsulated lipase (Ref. 20).
FIG. 3 View Enlargement | Download High Resolution Image (.zip file)
FIG. 4
Picture of κ-carrageenan encapsulated lipase in biodiesel production (a) before and (b) after use.
FIG. 4 View Enlargement | Download High Resolution Image (.zip file)

Tables

Table I. Comparison of process parameters in biodiesel production using Burkholderia cepacia lipase immobilized in various carrier matrices. [A—oil: alcohol molar ratio, B—optimum temperature (°C), C—optimum reaction time (h), and D—number of reuse with residual activity >65%.]
Table II. Reusability of κ-carrageenan encapsulated lipase in biodiesel production.

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